Podocyte-specific expression of Cre recombinase promotes glomerular basement membrane thickening
نویسندگان
چکیده
منابع مشابه
Podocyte-specific expression of tamoxifen-inducible Cre recombinase in mice.
BACKGROUND Podocytes play an important role in maintaining normal glomerular function. A podocyte-specific conditional knockout technology has been established by the use of transgenic mice expressing a podocyte-specific Cre recombinase to clarify the role of genes expressed in the podocytes. However, it may be difficult to examine the role of genes in certain pathologic conditions using conven...
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We report a transgenic mouse line that expresses Cre recombinase exclusively in podocytes. Twenty- four transgenic founders were generated in which Cre recombinase was placed under the regulation of a 2.5-kb fragment of the human NPHS2 promoter. Previously, this fragment was shown to drive beta-galactosidase (beta-gal) expression exclusively in podocytes of transgenic mice. For analysis, founde...
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Diffuse thickening of glomerular basement membranes (GBM) was optically observed in the renal grafts of 15 patients (21 specimens) among 70 recipients (128 specimens). Most of these specimens with diffuse GBM thickening were examined ultrastructurally. All cases with the above change were only found in allografts. The correlation was observed between the degree of GBM thickening and the clinica...
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Conventional silencing of many podocyte-specific genes in mice is associated with embryonic or perinatal lethality. Therefore, it would be of great importance to generate mouse models that allow the modification of genes that are expressed in podocytes at later stages of age. Herein is described a transgenic mouse with doxycycline-inducible podocyte-specific expression of Cre recombinase. For t...
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Evaluation of thick ascending limb (TAL) function has been hindered by the limited ability to selectively examine the function of this nephron segment in vivo. To address this, a Cre/loxP strategy was employed whereby the Tamm-Horsfall (THP) promoter was used to drive Cre recombinase expression in transgenic mice. The THP gene was cloned from a mouse genomic library, and 3.7 kb of the mouse THP...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Renal Physiology
سال: 2019
ISSN: 1931-857X,1522-1466
DOI: 10.1152/ajprenal.00359.2018